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rabbit anti mouse itk antibody  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rabbit anti mouse itk antibody
    Rabbit Anti Mouse Itk Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+mouse+itk+antibody/pmc12371038-87-37-41
    Average 86 stars, based on 1 article reviews
    rabbit anti mouse itk antibody - by Bioz Stars, 2026-11
    86/100 stars

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    Incubation:

    Article Title: ITK overexpression enhances T cell cytotoxicity against DLBCL through the TCR-Ca 2+ -Calcineurin-NFAT-IFN-γ pathway.
    Article Snippet: The proteins were then transferred to PVDF membranes (Millipore, USA, IPVH00010). .. The membranes were blocked with 5% non-fat milk (BD, USA, 232,100) in TBST buffer (20 mM Tris–HCl, 150 mM NaCl, 0.1% Tween 20, pH 7.6) for 1–2 h. The membranes were incubated overnight at 4 °C with rabbit anti-mouse ITK antibody (Cell Signaling Technology, USA, #3769) diluted 1:1000 and rabbit anti-mouse β-actin antibody (Cell Signaling Technology, USA, #4970) diluted 1:2000. .. HRP-labeled goat anti-rabbit secondary antibody (Cell Signaling Technology, USA, #7074) diluted 1:5000 was added and incubated at room temperature for 1–2 h. After washing with TBST, the membranes were developed using a chemiluminescent substrate (Thermo Fisher Scientific, USA, 34,580) and exposed on a chemiluminescent imaging system (Bio-Rad, USA, ChemiDoc MP).

    Article Title: ITK overexpression enhances T cell cytotoxicity against DLBCL through the TCR-Ca 2+ -Calcineurin-NFAT-IFN-γ pathway
    Article Snippet: The proteins were then transferred to PVDF membranes (Millipore, USA, IPVH00010). .. The membranes were blocked with 5% non-fat milk (BD, USA, 232,100) in TBST buffer (20 mM Tris–HCl, 150 mM NaCl, 0.1% Tween 20, pH 7.6) for 1–2 h. The membranes were incubated overnight at 4 °C with rabbit anti-mouse ITK antibody (Cell Signaling Technology, USA, #3769) diluted 1:1000 and rabbit anti-mouse β-actin antibody (Cell Signaling Technology, USA, #4970) diluted 1:2000. .. HRP-labeled goat anti-rabbit secondary antibody (Cell Signaling Technology, USA, #7074) diluted 1:5000 was added and incubated at room temperature for 1–2 h. After washing with TBST, the membranes were developed using a chemiluminescent substrate (Thermo Fisher Scientific, USA, 34,580) and exposed on a chemiluminescent imaging system (Bio-Rad, USA, ChemiDoc MP).



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